Home > Search Results: keywords:"izotermální amplifikace zprostředkovaná smyčkou (LAMP)"
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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Optimization of a loop-mediated isothermal amplification method for testing Streptococcus pneumoniae infection
 Friček, Matúš ; Jeřábek, Petr (advisor) ; Dračínská, Helena (referee)
Streptococcus pneumoniae is grampositive pathogen bacteria. According to World Health Organisation, diseases like pnemoniae or meningitidis are most commonly caused by S. pneumoniae infection. Gold standard for pathogen detection is currently polymerase chain reaction based on specific DNA amplification. During the last decades, many DNA amplification methods have been developed. One of them is loop mediated isothermal amplification (LAMP). Thanks to low instrumental requirements and simple interpretation, this method should be appropriate alternative for pathogen detection. Putting LAMP into clinical practise is complicated mainly because of common false positive results occurance in non DNA containing samples. Based on that, one of the aims of the thesis was to identificate and eliminate main sources of DNA contamination. According to the results, the main source of contamination seems to be the products from previous reactions. Nowdays, lytA and piaB genes are the most commonly used as targets for PCR detecion of S. pneumoniae. In available publications, there is not any mention about targetting LAMP method at piaB gene for detection of S. pneumoniae. That is why the aim of this bachelor thesis was to design optimal LAMP "primers" set for piaB gene detection. Within the optimization of the...
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